大菱鲆免疫球蛋白轻链IgL全长cDNA的分离、鉴定及表达分析     

王贤丽  张玉喜  孟亮  陈松林 《中国海洋大学学报(自然科学版)》2010,40(4)

实验采用末端快速扩增(RACE)技术从大菱鲆脾脏cDNA文库中筛选得到了免疫球蛋白轻链IgL的全长cD-NA片段。该序列包含47 bp的5′末端非编码区(5′UTR),738 bp的开放阅读框(ORF)和202 bp 3′UTR,整个开放阅读框编码246个氨基酸。系统发生分析表明大菱鲆IgL基因与五条鰤的IgL基因起源关系最近。RT-PCR分析表明,大菱鲆IgL基因只在正常脾脏、肾脏和头肾组织中表达;IgL在大菱鲆胚胎发育细胞期就已开始表达,在大菱鲆胚胎尾芽期和体节期表达持续增强;在鳗弧菌感染大菱鲆脾脏和头肾早期均检测到IgL基因强烈表达,后期表达逐渐减弱;大菱鲆胚胎细胞(TEC)在用鳗弧菌感染48h后,IgL开始表达。这些结果均表明IgL基因在大菱鲆免疫应答中起着重要作用。  相似文献   

粤东养殖区分离的2株海洋弧菌及其胞外产物对皱纹盘鲍致死毒性的初步分析     

房沙沙  林壮炳  邱礽  王劭雯  刘晓 《海洋科学》2013,37(8):16-22

2011年秋,福建和广东地区养殖的皱纹盘鲍(Haliotis discus hannai)相继暴发死亡。同年12月,我们从广东汕头病区取样分离得到2株弧菌,其中bb3为溶藻弧菌(Vibrio alginolyticus), bb4为哈氏弧菌(V. harveyi)。本研究以bb3、bb4作为供试菌株对1龄皱纹盘鲍进行侵染实验,获得如下结果：20℃条件下,供试菌及其胞外产物粗提液注射处理均可致受试鲍死亡,浓度2.5×107 cfu/mL 的 bb3与2.0×107cfu/mL的bb4悬液注射可分别引发43.33%和78.02%的受试鲍死亡;供试菌株的致死毒性与注射弧菌的总量正相关,与受试幼鲍的壳长、全湿重负相关;注射或浸泡-创伤两种处理方式均可导致受试鲍死亡,并且因注射供试弧菌而死亡的幼鲍软体部组织被健康鲍取食后也可引发取食者死亡;从侵染实验死亡的受试鲍软体部组织中可重新分离得到这2种供试弧菌。上述结果表明,溶藻弧菌 bb3和哈氏弧菌bb4是皱纹盘鲍的病原菌。药敏试验表明, bb3、bb4均已对抗生素具有一定程度的耐药性,在测试的20种抗生素中,仅有头孢曲松和丁胺卡那霉素是这2株弧菌同时高度敏感的抗生素,但bb3和bb4却对8种抗生素同时表现出抗性。  相似文献   

京津地区儿童呼吸系统疾病医疗气象预报初步研究   总被引：2，自引：0，他引：2  

张德山  刘燕  丁德平  张姝丽  穆启占  王燕  宛公展 《气候与环境研究》2007,12(6):804-810

通过研究京津地区儿童呼吸系统3种常见疾病(感冒、气管炎、肺炎)发病与环境气象条件的可能联系,发现气温、气温日较差和风速等气象要素与疾病发病率有一定的相关关系。依此,采用岭回归技术,建立了医疗气象预报模式。并用Visual Basic 6.0语言搭建了每日医疗气象滚动预报软件平台,可用于日常业务预报。  相似文献   

季节变化和气象要素变化与呼吸道疾病关系及预防措施初探     

苏斌 《气象研究与应用》2004,25(2):26-28

根据钦州市2003年气象要素及该市某综合医院2003年患呼吸道疾病的门诊住院病例医疗统计资料,分析季节变化及气象要素变化与呼吸道疾病的关系,提出合理利用气象条件对呼吸道疾病采取的预防保健措施。  相似文献   

Airline networks and the international diffusion of severe acute respiratory syndrome (SARS)   总被引：2，自引：0，他引：2  

JOHN T BOWEN JR    CHRISTIAN LAROE† 《The Geographical journal》2006,172(2):130-144

In fewer than four months in 2003, severe acute respiratory syndrome (SARS) spread from China to 25 countries and Taiwan, becoming the first new, easily transmissible infectious disease of the twenty-first century. The role of air transport in the diffusion of the disease became obvious early in the crisis; to assess that role more carefully, this study relates the spatial-temporal pattern of the SARS outbreak to a measure of airline network accessibility. Specifically, the accessibility from those countries that were infected by SARS, beginning with China, to other countries was measured using airline schedules. The country-pair accessibility measure, along with other country-level factors relevant to the disease, were tested as determinants of the speed with which SARS arrived in infected countries as well as its failure to arrive in most countries. The analyses indicate that airline network accessibility was an especially influential variable but also that the importance of this variable diminished in the latter weeks of the outbreak. The latter finding is partly attributable to public health measures, particularly health screening in airports. The timing and geography of those measures are reviewed using data from media reports and interim World Health Organization (WHO) documents during the outbreak. The uneven effort to curtail the international diffusion of SARS suggests further planning is needed to develop a concerted response to contain future epidemics.  相似文献   

The 1985 outburst of RS Ophiuchi: Spectroscopic results     

G. C. Anupama  T. P. Prabhu 《Journal of Astrophysics and Astronomy》1989,10(3):237-255

Optical spectroscopic data on the recurrent nova RS Ophiuchi obtained between 32 and 108 days after its last outburst on 1985 January 27 are presented. RS Oph was in the coronal-line phase at that time. The widths of the permitted as well as coronal-lines decreased continuously. Assuming that the ejected envelope decelerated due to its interaction with circum stellar matter, its size is deduced as a function of time. Observed fluxes in permitted lines would then imply that the electron density decreased from 3 × 10⁹ cm^#x2212;3 on day 32 to 1.8 × 10⁸ cm^-3 on day 108, for an assumed filling factor of 0.01. The helium abundance in the ejecta is estimated to be n(He)/n(H) ∼ 0.16. The mass of the unshocked ejecta was 3 × 10-6 (Φ/0.01)^1/2 M_⊙, (at this stage, where f is the filling factor. Observed fluxes in coronal-lines imply that the temperature of coronal-line region decreased from 1.5 × 10⁶ K on day 32 to 1.1 × 10⁶ K on day 108. Most of the coronal line emission, as well as He n emission arises in shocked and cooling ejecta. This region is not isothermal, but contains material at a wide range of temperatures. Mass of the shocked ejecta is estimated to be in the range 10⁻⁷−10⁻⁶ M⊙ Based on the number of H- and He-ionizing photons, we estimate that the ionizing source evolved from a radius and temperature of (2 × l0¹² cm, 3 × 10⁴ K) on day 32 to (6 × l0⁹ cm, 3.6 × 10⁵K) on day 204. We also present the spectra of RS Oph recorded in quiescent phase, 2 and 3 years after outburst, for comparison. The spectrum is dominated by that of M2 giant secondary, with superposed emission lines of H and He I  相似文献   

A spectroscopic study of the envelope of the recurrent Nova CI Aquilae     

M. A. Burlak  V. F. Esipov 《Astronomy Letters》2001,27(9):574-580

Spectroscopic observations of the recurrent Nova CI Aql in the wavelength range 4000–11000 Å are presented. Its evolution is traced from maximum light to the disappearance of nebular lines. 〈E _B-V 〉 = 0.91 ± 0.11, as inferred from the Balmer decrement. The mean expansion velocity of the envelope measured near maximum light is 2800 km s^?1. The helium abundance in the Nova envelope has been found to be enhanced, ?He/H?=0.22. CI Aql is similar in spectral evolution, in change of the envelope expansion velocity, and in helium abundance to other recurrent novae.  相似文献   

肠道病毒病毒感染的季节和年龄分布特征及其与气象条件的分析     

席林华  刘培宁 《气象科学》2001,21(3):374-378

本文利用苏州市二院和苏州中医院1990-1995年住院病例资料，研究肠道感染，病毒感染的年龄、季节分布特征及其肠道发病住院与气象要素变化的关系，以求探索一定规律、指标供防病保健对策应用。  相似文献   

论工程场地地震安全性评价中历史地震影响烈度的分析方法──以南澳跨海大桥为例     

贾素娟 《中国地震》1996,(4)

合理地确定场地的历史地震影响烈度，是工程场地地震安全性评价工作中的重要一环。本文以广东南澳跨海大桥场址为例，论述了历史地震影响烈度分析的要点。首先对南澳附近１９１８年７．３级地震的震中位置及对桥址的影响烈度进行了确认；其次对选择合适的区域地震烈度衰减关系进行了分析比较，并建立了合理的桥址影响烈度目录；最后应用极值统计分析方法，得到不同平均重现期所对应的烈度值，为大桥的抗震设计提供了设防依据。  相似文献   

Integrated Spatio‐temporal Data Mining for Forest Fire Prediction     

Tao Cheng  Jiaqiu Wang 《Transactions in GIS》2008,12(5):591-611

Forests play a critical role in sustaining the human environment. Most forest fires not only destroy the natural environment and ecological balance, but also seriously threaten the security of life and property. The early discovery and forecasting of forest fires are both urgent and necessary for forest fire control. This article explores the possible applications of Spatio‐temporal Data Mining for forest fire prevention. The research pays special attention to the spatio‐temporal forecasting of forest fire areas based upon historic observations. An integrated spatio‐temporal forecasting framework – ISTFF – is proposed: it uses a dynamic recurrent neural network for spatial forecasting. The principle and algorithm of ISTFF are presented, and are then illustrated by a case study of forest fire area prediction in Canada. Comparative analysis of ISTFF with other methods shows its high accuracy in short‐term prediction. The effect of spatial correlations on the prediction accuracy of spatial forecasting is also explored.  相似文献